Digital DNA-seq technology – targeted enrichment for cancer research
Targeted DNA sequencing has become a powerful approach as it achieves high coverage of the region of interest, while keeping the cost of sequencing and complexity of data interpretation manageable. However, existing PCR-based target enrichment approaches introduce errors due to PCR amplification bias and artifacts, which significantly affect quantification accuracy and limit the ability to confidently detect low-frequency DNA variants. This webinar introduces a new digital sequencing approach using QIAseq Targeted DNA Panels, which are based on Unique Molecular Indices (UMI) technology. With UMIs, each unique DNA molecule is barcoded before any amplification takes place to correct for PCR errors. Join us and learn about this exciting and novel digital DNA-seq technology. Detailed workflow and applications in cancer research will be presented.
Targeted DNA sequencing has become a powerful approach as it achieves high coverage of the region of interest, while keeping the cost of sequencing and complexity of data interpretation manageable. However, existing PCR-based target enrichment approaches introduce errors due to PCR amplification bias and artifacts, which significantly affect quantification accuracy and limit the ability to confidently detect low-frequency DNA variants. This webinar introduces a new digital sequencing approach using QIAseq Targeted DNA Panels, which are based on Unique Molecular Indices (UMI) technology. With UMIs, each unique DNA molecule is barcoded before any amplification takes place to correct for PCR errors. Join us and learn about this exciting and novel digital DNA-seq technology. Detailed workflow and applications in cancer research will be presented.
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